Mutations in the CDP-choline pathway for phospholipid biosynthesis bypass the requirement for an essential phospholipid transfer protein

Cell. 1991 Feb 22;64(4):789-800. doi: 10.1016/0092-8674(91)90508-v.


SEC14p is the yeast phosphatidylinositol (PI)/phosphatidylcholine (PC) transfer protein, and it effects an essential stimulation of yeast Golgi secretory function. We now report that the SEC14p localizes to the yeast Golgi and that the SEC14p requirement can be specifically and efficiently bypassed by mutations in any one of at least six genes. One of these suppressor genes was the structural gene for yeast choline kinase (CKI), disruption of which rendered the cell independent of the normally essential SEC14p requirement. The antagonistic action of the CKI gene product on SEC14p function revealed a previously unsuspected influence of biosynthetic activities of the CDP-choline pathway for PC biosynthesis on yeast Golgi function and indicated that SEC14p controls the phospholipid content of yeast Golgi membranes in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • Cytidine Diphosphate Choline / metabolism*
  • Genes, Fungal*
  • Genes, Suppressor*
  • Genotype
  • Golgi Apparatus / metabolism
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Phosphatidylcholines / metabolism
  • Phosphatidylinositols / metabolism
  • Phospholipid Transfer Proteins*
  • Phospholipids / biosynthesis*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Suppression, Genetic


  • Carrier Proteins
  • Membrane Proteins
  • Phosphatidylcholines
  • Phosphatidylinositols
  • Phospholipid Transfer Proteins
  • Phospholipids
  • Cytidine Diphosphate Choline