Interleukin-8 induces neutrophil transendothelial migration

Immunology. 1991 Jan;72(1):65-72.


Interleukin-8 (IL-8) is a potent neutrophil chemotactic stimulant. We have used chemically synthesized IL-8 to investigate its role in human neutrophil adhesion and transendothelial migration. IL-8 enhanced the adhesiveness of human neutrophils to plastic, and to both unstimulated and tumour necrosis factor (TNF)-stimulated endothelial monolayers in vitro. Using a two-compartment model separated by a confluent endothelial monolayer, we have shown that IL-8 chemotactic stimulation induced transmigration across the monolayer of up to 87.4 +/- 2.1% of added neutrophils (compared to random unstimulated transmigration of 2.2 +/- 0.7%), while chemokinetic stimulation led to transmigration of 21 +/- 3.8% of neutrophils. Preincubation of endothelium with TNF also induced transmigration in this model, and was additive when combined with an IL-8 chemotactic stimulus. Endothelial permeability was increased at maximal rates of chemotactic transmigration, which may correlate with increased permeability of vessels at inflammatory sites in vivo. The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capillary Permeability
  • Cell Adhesion
  • Cell Movement
  • Chemotactic Factors / physiology*
  • Endothelium, Vascular / metabolism
  • Humans
  • Interleukin-8 / physiology*
  • Neutrophils / physiology*
  • Polystyrenes
  • Tumor Necrosis Factor-alpha / physiology
  • Umbilical Veins / metabolism


  • Chemotactic Factors
  • Interleukin-8
  • Polystyrenes
  • Tumor Necrosis Factor-alpha