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Review
. 2010 Jan;11(1):31-46.
doi: 10.1038/nrg2626. Epub 2009 Dec 8.

Sequencing Technologies - The Next Generation

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Review

Sequencing Technologies - The Next Generation

Michael L Metzker. Nat Rev Genet. .

Abstract

Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest.

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