Synthesis and screening of a CaaL peptide library versus FTase reveals a surprising number of substrates

Bioorg Med Chem Lett. 2010 Jan 15;20(2):767-70. doi: 10.1016/j.bmcl.2009.11.011. Epub 2009 Nov 12.

Abstract

Proteins bearing a CaaL sequence are typically geranylgeranylated to enable their proper localization and function. We found that many of the dansyl-GCaaL peptides representing mammalian CaaL proteins can be farnesylated by FTase. This result may have important implications for prenylated protein biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / metabolism
  • Amino Acid Sequence
  • Farnesyltranstransferase / metabolism*
  • Peptide Library
  • Peptides / chemical synthesis*
  • Peptides / chemistry
  • Peptides / pharmacology
  • Prenylation
  • Substrate Specificity
  • ras Proteins / metabolism

Substances

  • Peptide Library
  • Peptides
  • Alkyl and Aryl Transferases
  • geranylgeranyltransferase type-I
  • Farnesyltranstransferase
  • ras Proteins