Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain

Biochem Biophys Res Commun. 2010 Jan 15;391(3):1348-51. doi: 10.1016/j.bbrc.2009.12.050. Epub 2009 Dec 16.


The functional role of CD36 protein detected in mitochondrial fractions in long chain fatty acid (LCFA) oxidation is unclear due to conflicting results obtained in Cd36 knockout mice and experiments using sulfo-N-succinimidyl oleate (SSO) for inhibition of CD36 mediated LCFA transport. We investigated effect of SSO on mitochondrial respiration and found that SSO substantially inhibits not only LCFA oxidation, but also oxidation of flavoprotein- and NADH-dependent substrates and generation of mitochondrial membrane potential. Experiments in rat liver, heart and kidney mitochondria demonstrated a direct effect on mitochondrial respiratory chain with the most pronounced inhibition of the complex III (IC(50) 4microM SSO). The results presented here show that SSO is a potent and irreversible inhibitor of mitochondrial respiratory chain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • CD36 Antigens / drug effects*
  • CD36 Antigens / genetics
  • CD36 Antigens / metabolism
  • Cell Respiration / drug effects
  • Electron Transport Complex III / antagonists & inhibitors*
  • Fatty Acids / metabolism*
  • Male
  • Membrane Potential, Mitochondrial / drug effects
  • Mice
  • Mice, Knockout
  • Mitochondria / drug effects*
  • Mitochondria / enzymology
  • Oleic Acids / pharmacology*
  • Rats
  • Rats, Inbred WKY
  • Succinimides / pharmacology*


  • CD36 Antigens
  • Fatty Acids
  • Oleic Acids
  • Succinimides
  • sulfo-N-succinimidyl oleate
  • Electron Transport Complex III