Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation

Am J Physiol Renal Physiol. 2010 Mar;298(3):F502-9. doi: 10.1152/ajprenal.00476.2009. Epub 2009 Dec 9.


Vasopressin influences salt and water transport in renal epithelia. This is coordinated by the combined action of V2 receptor-mediated effects along distinct nephron segments. Modulation of NaCl reabsorption by vasopressin has been established in the loop of Henle, but its role in the distal convoluted tubule (DCT), an effective site for fine regulation of urinary electrolyte composition and the target for thiazide diuretics, is largely unknown. The Na+-Cl- cotransporter (NCC) of DCT is activated by luminal trafficking and phosphorylation at conserved NH2-terminal residues. Here, we demonstrate the effects of short-term vasopressin administration (30 min) on NCC activation in Brattleboro rats with central diabetes insipidus (DI) using the V2 receptor agonist desmopressin (dDAVP). The fraction of NCC abundance in the luminal plasma membrane was significantly increased upon dDAVP as shown by confocal microscopy, immunogold cytochemistry, and Western blot, suggesting increased apical trafficking of the transporter. Changes were paralleled by augmented phosphorylation of NCC as detected by antibodies against phospho-threonine and phospho-serine residues (2.5-fold increase at Thr53 and 1.4-fold increase at Ser71). dDAVP-induced phosphorylation of NCC, studied in tubular suspensions in the absence of systemic effects, was enhanced as well (1.7-fold increase at Ser71), which points to the direct mode of action of vasopressin in DCT. Changes were more pronounced in early (DCT1) than in late DCT as distinguished by the distribution of 11beta-hydroxysteroid dehydrogenase 2 in DCT2. These results suggest that the vasopressin-V(2) receptor-NCC signaling cascade is a novel effector system to adjust transepithelial NaCl reabsorption in DCT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 11-beta-Hydroxysteroid Dehydrogenase Type 2 / metabolism
  • Animals
  • Antidiuretic Agents / administration & dosage*
  • Blotting, Western
  • Cell Membrane / drug effects*
  • Cell Membrane / metabolism
  • Cell Membrane / ultrastructure
  • Chlorides / metabolism
  • Deamino Arginine Vasopressin / administration & dosage*
  • Diabetes Insipidus, Neurogenic / drug therapy
  • Diabetes Insipidus, Neurogenic / metabolism*
  • Diabetes Insipidus, Neurogenic / pathology
  • Diabetes Insipidus, Neurogenic / physiopathology
  • Disease Models, Animal
  • Immunohistochemistry
  • Kidney Tubules, Distal / drug effects*
  • Kidney Tubules, Distal / metabolism
  • Kidney Tubules, Distal / physiopathology
  • Kidney Tubules, Distal / ultrastructure
  • Male
  • Microscopy, Confocal
  • Natriuresis / drug effects
  • Phosphorylation
  • Protein Transport
  • Rats
  • Rats, Brattleboro
  • Rats, Wistar
  • Receptors, Drug / drug effects*
  • Receptors, Drug / metabolism
  • Receptors, Vasopressin / agonists*
  • Receptors, Vasopressin / metabolism
  • Sodium / metabolism
  • Sodium Chloride Symporter Inhibitors / pharmacology*
  • Solute Carrier Family 12, Member 3
  • Symporters / drug effects*
  • Symporters / metabolism
  • Time Factors
  • Up-Regulation


  • Antidiuretic Agents
  • Chlorides
  • Receptors, Drug
  • Receptors, Vasopressin
  • Slc12a3 protein, rat
  • Sodium Chloride Symporter Inhibitors
  • Solute Carrier Family 12, Member 3
  • Symporters
  • Sodium
  • 11-beta-Hydroxysteroid Dehydrogenase Type 2
  • Deamino Arginine Vasopressin