Poly(vinyl alcohol)/halloysite nanotubes bionanocomposite films: Properties and in vitro osteoblasts and fibroblasts response

J Biomed Mater Res A. 2010 Jun 15;93(4):1574-87. doi: 10.1002/jbm.a.32656.


In this study, transparent poly(vinyl alcohol) (PVA) and PVA/halloysite nanotubes (HNTs) bionanocomposite films were prepared by solution casting and glutaraldehyde (GA) crosslinking. The surface topography and chemistry of the films were characterized by atomic force microscopy (AFM) and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, respectively. Blending with HNTs induced changes in nanotopography and surface chemistry of PVA films. The mechanical properties of PVA were enhanced by the incorporated HNTs. The stain-induced crystallization was confirmed by DSC after tensile test. MC3T3-E1 osteoblast-like and NIH 3T3 fibroblast cells were cultured on neat PVA and PVA/HNTs films to evaluate the effects of surface nanotopography and composition on cell behavior. The observations indicated that MC3T3-E1 cell behavior strongly responded to surface nanotopography. On nanotube-dominant surface, cells exhibited a significantly higher level of adhesion than on neat PVA film, whereas neat PVA showed higher degree of osteoblast proliferation compared with PVA/HNTs. In vitro fibroblasts response demonstrated that both neat PVA and PVA/HNTs nanocomposite films were biocompatible and PVA/HNTs films favored to fibroblasts attach and growth below 7.5 wt % of HNTs incorporated. In summary, these results provided insights into understanding of PVA and PVA/HNTs bionanocomposite films in potential applications in bone tissue engineering and drug delivery systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Aluminum Silicates / chemistry*
  • Animals
  • Biocompatible Materials / chemistry*
  • Bone and Bones / metabolism
  • Clay
  • Cross-Linking Reagents / chemistry
  • Drug Delivery Systems
  • Fibroblasts / cytology*
  • Glutaral / chemistry
  • Mice
  • Microscopy, Atomic Force / methods
  • Nanocomposites / chemistry*
  • Nanotechnology / methods*
  • Nanotubes / chemistry*
  • Osteoblasts / cytology*
  • Polyvinyl Alcohol / chemistry*
  • Spectroscopy, Fourier Transform Infrared / methods
  • Surface Properties
  • Tissue Engineering / methods


  • Aluminum Silicates
  • Biocompatible Materials
  • Cross-Linking Reagents
  • Polyvinyl Alcohol
  • Clay
  • Glutaral