Intraspecies diversity of SARS-like coronaviruses in Rhinolophus sinicus and its implications for the origin of SARS coronaviruses in humans

J Gen Virol. 2010 Apr;91(Pt 4):1058-62. doi: 10.1099/vir.0.016378-0. Epub 2009 Dec 16.

Abstract

The Chinese rufous horseshoe bat (Rhinolophus sinicus) has been suggested to carry the direct ancestor of severe acute respiratory syndrome (SARS) coronavirus (SCoV), and the diversity of SARS-like CoVs (SLCoV) within this Rhinolophus species is therefore worth investigating. Here, we demonstrate the remarkable diversity of SLCoVs in R. sinicus and identify a strain with the same pattern of phylogenetic incongruence (i.e. an indication of recombination) as reported previously in another SLCoV strain. Moreover, this strain possesses a distinctive 579 nt deletion in the nsp3 region that was also found in a human SCoV from the late-phase epidemic. Phylogenetic analysis of the Orf1 region suggested that the human SCoVs are phylogenetically closer to SLCoVs in R. sinicus than to SLCoVs in other Rhinolophus species. These findings reveal a closer evolutionary linkage between SCoV in humans and SLCoVs in R. sinicus, defining the scope of surveillance to search for the direct ancestor of human SCoVs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chiroptera / virology*
  • Cytochromes b / genetics
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • RNA Replicase / genetics
  • Recombination, Genetic
  • SARS Virus / classification*
  • SARS Virus / genetics
  • Viral Nonstructural Proteins / genetics

Substances

  • Viral Nonstructural Proteins
  • Cytochromes b
  • Nsp3 protein, SARS-CoV
  • RNA Replicase

Associated data

  • GENBANK/FJ561748
  • GENBANK/FJ588681
  • GENBANK/FJ588682
  • GENBANK/FJ588683
  • GENBANK/FJ588684
  • GENBANK/FJ588685
  • GENBANK/FJ588686
  • GENBANK/FJ588687
  • GENBANK/FJ588688
  • GENBANK/FJ588689
  • GENBANK/FJ588690
  • GENBANK/FJ588691
  • GENBANK/FJ588692