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, 279 (2), 210-5

Cloning and Sequencing of Lol pI, the Major Allergenic Protein of Rye-Grass Pollen

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Cloning and Sequencing of Lol pI, the Major Allergenic Protein of Rye-Grass Pollen

I J Griffith et al. FEBS Lett.

Abstract

We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.

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