Cloning and sequencing of Lol pI, the major allergenic protein of rye-grass pollen

FEBS Lett. 1991 Feb 25;279(2):210-5. doi: 10.1016/0014-5793(91)80151-r.


We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens*
  • Amino Acid Sequence
  • Antigens, Plant
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Genes, Plant
  • Glycoproteins / genetics
  • Molecular Sequence Data
  • Plant Proteins*
  • Pollen* / genetics*
  • Secale / genetics*
  • Secale / immunology


  • Allergens
  • Antigens, Plant
  • Glycoproteins
  • Lol p I protein, Lolium perenne
  • Plant Proteins
  • DNA

Associated data

  • GENBANK/X57678
  • GENBANK/X58797
  • GENBANK/X58798
  • GENBANK/X58799
  • GENBANK/X58800
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X62840
  • GENBANK/X62841