Abstract
Aim of the study:
The present study was designed to investigate effect of guggulipid, a drug developed by CDRI and nimesulide on LPS stimulated neuroinflammatory changes in rat astrocytoma cell line (C6).
Materials and methods:
Rat astrocytoma cells (C6) were stimulated with LPS (10 microg/ml) alone and in combinations with different concentrations of guggulipid or nimesulide for 24h of incubation. Nitrite release in culture supernatant, ROS in cells, expressions of COX-2, GFAP and TNF-alpha in cell lysate were estimated.
Results:
LPS (10 microg/ml) stimulated C6 cells to release nitrite, ROS generation, up regulated COX-2 and GFAP expressions at protein level and TNF-alpha at mRNA level. Both guggulipid and nimesulide significantly attenuated nitrite release, ROS generation and also down regulated expressions of COX-2, GFAP and TNF-alpha. Guggulipid and nimesulide per se did not have any significant effect on C6 cells.
Conclusion:
Results demonstrate the anti-inflammatory effect of guggulipid comparable to nimesulide which suggest potential use of guggulipid in neuroinflammation associated conditions in CNS disorders.
Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / pharmacology*
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Anti-Inflammatory Agents / therapeutic use
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Antioxidants / pharmacology
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Antioxidants / therapeutic use
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Astrocytoma / immunology
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Astrocytoma / metabolism*
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Cell Line, Tumor
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Commiphora
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Cyclooxygenase 2 / genetics
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Cyclooxygenase 2 / metabolism
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Down-Regulation
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Gene Expression
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Glial Fibrillary Acidic Protein / genetics
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Glial Fibrillary Acidic Protein / metabolism*
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Inflammation / metabolism
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Inflammation / prevention & control*
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Inflammation Mediators / metabolism*
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Lipopolysaccharides
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Nitrites / metabolism
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Phytotherapy
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Plant Gums / pharmacology*
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Plant Gums / therapeutic use
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RNA, Messenger / metabolism
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Rats
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Reactive Oxygen Species / metabolism
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Sulfonamides / pharmacology
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Sulfonamides / therapeutic use*
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Anti-Inflammatory Agents
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Antioxidants
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Glial Fibrillary Acidic Protein
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Inflammation Mediators
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Lipopolysaccharides
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Nitrites
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Plant Extracts
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Plant Gums
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RNA, Messenger
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Reactive Oxygen Species
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Sulfonamides
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Tumor Necrosis Factor-alpha
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guggulu extract
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Cyclooxygenase 2
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nimesulide