Effect of pomegranate juice on Angiotensin II-induced hypertension in diabetic Wistar rats

Phytother Res. 2010 Jun;24 Suppl 2:S196-203. doi: 10.1002/ptr.3090.


Acute subcutaneous administration of Angiotensin II (Ang II) causes a rise in blood pressure in diabetic Wistar rats. Diabetes was induced using streptozotocin (70 mg/kg, i.v.). Chronic administration of pomegranate juice (PJ) extract (100 mg/kg and 300 mg/kg; p.o. for 4 weeks) obtained from Punica granatum (punicaceae) fruits reduced the mean arterial blood pressure and vascular reactivity changes to various catecholamines and also reversed the biochemical changes induced by diabetes and Ang II. PJ treatment also caused a significant decrease in levels of thiobarbituric acid reactive substances (TBARS) in kidney and pancreas while activities of enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH) showed significant elevation. The cumulative concentration response curve (CCRC) of Ang II was shifted towards right in rats treated with PJ using isolated strip of ascending colon. In histopathological examination, PJ treatment prevented the tubular degenerative changes induced by diabetes. The results suggest that the PJ extract could prevent the development of high blood pressure induced by Ang II in diabetic rats probably by combating the oxidative stress induced by diabetes and Ang II and by inhibiting ACE activity. In conclusion, PJ has antihypertensive action in Ang II diabetic model.

MeSH terms

  • Angiotensin II / pharmacology
  • Animals
  • Blood Pressure / drug effects
  • Catalase / metabolism
  • Colon / drug effects
  • Colon / pathology
  • Diabetes Mellitus, Experimental / drug therapy*
  • Fruit / chemistry*
  • Glutathione Reductase / metabolism
  • Hypertension / drug therapy*
  • Lythraceae / chemistry*
  • Male
  • Oxidative Stress / drug effects
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism


  • Thiobarbituric Acid Reactive Substances
  • Angiotensin II
  • Catalase
  • Superoxide Dismutase
  • Glutathione Reductase