Abstract
The ability of Saccharomyces cerevisiae transcription factors IIIC and IIIB to bend DNA at their binding sites in a tRNA(Gln) gene has been analyzed by two methods that are based on the anomalous electrophoretic mobility of bent DNA in a tight gel network. Both transcription factors bend DNA, TFIIIB more than TFIIIC. The effective centers of the transcription factor IIIB- and IIIC-induced bends are not in helical phase.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Autoradiography
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Base Sequence
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Binding Sites
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DNA*
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Electrophoresis, Polyacrylamide Gel
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Fungal Proteins / metabolism*
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Molecular Sequence Data
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Nucleic Acid Conformation
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Plasmids / genetics
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RNA, Transfer, Gln / genetics*
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Saccharomyces cerevisiae / metabolism*
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Transcription Factor TFIIIB
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Transcription Factors / metabolism*
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Transcription Factors, TFIII*
Substances
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Fungal Proteins
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RNA, Transfer, Gln
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Transcription Factor TFIIIB
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Transcription Factors
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Transcription Factors, TFIII
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transcription factor TFIIIC
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DNA