Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene

J Biol Chem. 1991 Mar 15;266(8):5162-8.

Abstract

The ability of Saccharomyces cerevisiae transcription factors IIIC and IIIB to bend DNA at their binding sites in a tRNA(Gln) gene has been analyzed by two methods that are based on the anomalous electrophoretic mobility of bent DNA in a tight gel network. Both transcription factors bend DNA, TFIIIB more than TFIIIC. The effective centers of the transcription factor IIIB- and IIIC-induced bends are not in helical phase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoradiography
  • Base Sequence
  • Binding Sites
  • DNA*
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / metabolism*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Plasmids / genetics
  • RNA, Transfer, Gln / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Transcription Factor TFIIIB
  • Transcription Factors / metabolism*
  • Transcription Factors, TFIII*

Substances

  • Fungal Proteins
  • RNA, Transfer, Gln
  • Transcription Factor TFIIIB
  • Transcription Factors
  • Transcription Factors, TFIII
  • transcription factor TFIIIC
  • DNA