Exercise improvement and plasma biomarker changes with intravenous treprostinil therapy for pulmonary arterial hypertension: a placebo-controlled trial

J Heart Lung Transplant. 2010 Feb;29(2):137-49. doi: 10.1016/j.healun.2009.09.005.


Background: Pulmonary arterial hypertension (PAH) remains a poorly understood and frequently lethal disease with few treatment options.

Methods: We conducted a placebo-controlled trial of intravenous treprostinil, a prostacyclin analog, in treatment-naive PAH patients. During 12 weeks of treatment with treprostinil or placebo, we quantified 6-minute walk distance (6MW), clinical symptoms and 11 cytokines/growth factors.

Results: Forty-two of 44 study patients had idiopathic/familial PAH in New York Heart Association (NYHA) Class III. Treprostinil increased 6MW by a placebo-corrected median of 83 meters (p = 0.008; mean increase 93 +/- 42 meters), reduced Borg score by a median 2.0 units (p = 0.02), and improved NYHA class by a median of 1.0 (p = 0.02). There was a trend toward improved survival with treprostinil (p = 0.051). Baseline plasma angiopoietin-2 (Ang-2), vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) and platelet-derived growth factor (PDGF) were elevated compared with reported normal ranges. Treatment with treprostinil was associated with decreased Ang-2 levels. Improvement in 6MW distance after treatment was associated with reductions in Ang-2 and MMP-9 levels. Most of the cytokines and growth factors studied were not abnormal with disease nor did they change with treatment.

Conclusions: We conclude that treprostinil treatment significantly improved exercise capacity, dyspnea and functional class. Several plasma proteins that might track disease were abnormal at baseline, and changes were associated with improved exercise capacity.

Trial registration: ClinicalTrials.gov NCT00494533.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antihypertensive Agents / administration & dosage*
  • Biomarkers / blood
  • Double-Blind Method
  • Epoprostenol / administration & dosage
  • Epoprostenol / analogs & derivatives*
  • Exercise
  • Female
  • Humans
  • Hypertension, Pulmonary / drug therapy*
  • Infusions, Intravenous
  • Male
  • Middle Aged
  • Physical Endurance / drug effects*
  • Severity of Illness Index
  • Treatment Outcome
  • Walking


  • Antihypertensive Agents
  • Biomarkers
  • Epoprostenol
  • treprostinil

Associated data

  • ClinicalTrials.gov/NCT00494533