Isolation and immunological characterization of a novel Cladosporium herbarum allergen structurally homologous to the alpha/beta hydrolase fold superfamily

Mol Immunol. 2010 Mar;47(6):1366-77. doi: 10.1016/j.molimm.2009.11.027. Epub 2009 Dec 22.


Because the ascomycete Cladosporium herbarum embodies one of the most important, world-wide occurring fungal species responsible for eliciting typical IgE-mediated hypersensitivity reactions ranging from rhinitis and ocular symptoms to severe involvement of the lower respiratory tract, a more comprehensive definition of its detailed allergen repertoire is unquestionably of critical medical as well as therapeutic significance. By screening a C. herbarum cDNA library with IgE antibodies pooled from 3 mold-reactive sera, we were able to identify, clone and affinity-purify a novel allergen candidate (29.9 kDa) exhibiting considerable (three-dimensional) homology to the alpha/beta hydrolase fold superfamily. The latter covers a collection of hydrolytic enzymes of widely differing phylogenetic origin as well as catalytic activity (operating in countless biological contexts) that in general exhibit only little sequence similarity yet show a remarkable conservation of structural topology. Our present study (i) characterizes recombinant non-fusion C. herbarum hydrolase as a natively folded, minor mold allergen that displays a prevalence of IgE reactivity of approximately 17% in our in vitro immunoblot experiments, (ii) proposes the existence of several putative (speculatively cross-reactive) ascomycete orthologues as determined via genome-wide in silico predictions, and (iii) finally implies that C. herbarum hydrolase could be included in forthcoming minimal testing sets when fungal allergy is suspected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / chemistry
  • Allergens / genetics
  • Allergens / immunology*
  • Allergens / isolation & purification*
  • Amino Acid Sequence
  • Base Sequence
  • Cladosporium / enzymology*
  • Cladosporium / immunology*
  • Humans
  • Hydrolases / chemistry
  • Hydrolases / genetics
  • Hydrolases / immunology*
  • Hydrolases / isolation & purification*
  • Immunoblotting
  • Immunoglobulin E / immunology
  • Models, Molecular
  • Molecular Sequence Data
  • Multigene Family
  • Protein Renaturation
  • Recombinant Proteins / immunology
  • Sequence Alignment
  • Structural Homology, Protein*


  • Allergens
  • Recombinant Proteins
  • Immunoglobulin E
  • Hydrolases

Associated data

  • GENBANK/DQ159861