Using BCG, MPT-51 and Ag85 as antigens in an indirect ELISA for the diagnosis of bovine tuberculosis

Vet J. 2011 Feb;187(2):276-8. doi: 10.1016/j.tvjl.2009.11.017. Epub 2009 Dec 22.


This study evaluated the Mycobacterium tuberculosis protein antigen MPT-51, the trimeric antigen 85 (Ag85) complex, and Bacillus Calmette-Guérin (BCG) in an indirect ELISA to diagnose bovine tuberculosis (TB) from serum samples. Serum was collected from 208 intra-dermal tuberculin test (ITT)-positive and 54 ITT-negative animals from a region where bovine TB is endemic. Using the Ag85 and BCG antigens, the indirect ELISA was able to discriminate ITT-positive from ITT-negative animals. This level of discrimination was not achieved when using the MPT-51 antigen. The highest sensitivity (Se) and specificity (Sp) of the test was found when BCG was used (Se, 82%; Sp, 91%). Further work in different epidemiological settings and with larger numbers of animals will be required to validate these findings.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / blood
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins / blood
  • Bacterial Proteins / immunology*
  • Cattle
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Mycobacterium bovis / immunology*
  • Sensitivity and Specificity
  • Tuberculin Test / veterinary
  • Tuberculosis, Bovine / blood
  • Tuberculosis, Bovine / diagnosis*


  • Antigens, Bacterial
  • Bacterial Proteins
  • MPT51 protein, Mycobacterium
  • antigen 85, Mycobacterium bovis
  • Mycobacterium tuberculosis antigens