Developmental dynamics of cone photoreceptors in the eel

BMC Dev Biol. 2009 Dec 21;9:71. doi: 10.1186/1471-213X-9-71.

Abstract

Background: Many fish alter their expressed visual pigments during development. The number of retinal opsins expressed and their type is normally related to the environment in which they live. Eels are known to change the expression of their rod opsins as they mature, but might they also change the expression of their cone opsins?

Results: The Rh2 and Sws2 opsin sequences from the European Eel were isolated, sequenced and expressed in vitro for an accurate measurement of their lambdamax values. In situ hybridisation revealed that glass eels express only rh2 opsin in their cone photoreceptors, while larger yellow eels continue to express rh2 opsin in the majority of their cones, but also have <5% of cones which express sws2 opsin. Silver eels showed the same expression pattern as the larger yellow eels. This observation was confirmed by qPCR (quantitative polymerase chain reaction).

Conclusions: Larger yellow and silver European eels express two different cone opsins, rh2 and sws2. This work demonstrates that only the Rh2 cone opsin is present in younger fish (smaller yellow and glass), the sws2 opsin being expressed additionally only by older fish and only in <5% of cone cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cone Opsins / genetics
  • Cone Opsins / metabolism*
  • Eels / embryology*
  • Eels / genetics
  • Fish Proteins / genetics
  • Fish Proteins / metabolism*
  • Gene Expression Regulation, Developmental*
  • Photoreceptor Cells, Vertebrate
  • Phylogeny
  • Retina / embryology*
  • Retinal Cone Photoreceptor Cells / metabolism*
  • Retinal Pigments / metabolism

Substances

  • Cone Opsins
  • Fish Proteins
  • Retinal Pigments