Effects of oral creatine and resistance training on serum myostatin and GASP-1

Mol Cell Endocrinol. 2010 Apr 12;317(1-2):25-30. doi: 10.1016/j.mce.2009.12.019. Epub 2009 Dec 22.


Myostatin is a catabolic regulator of skeletal muscle mass. The purpose of this study was to determine the effect of resistance training for 8 weeks in conjunction with creatine supplementation on muscle strength, lean body mass, and serum levels of myostatin and growth and differentiation factor-associated serum protein-1 (GASP-1). In a double-blinded design 27 healthy male subjects (23.42+/-2.2 years) were assigned to control (CON), resistance training+placebo (RT+PL) and resistance training+creatine supplementation (RT+CR) groups. The protocol consisted of 3 days per week of training for 8 weeks, each session including three sets of 8-10 repetitions at 60-70% of 1 RM for whole-body exercise. Blood sampling, muscular strength testing and body composition analysis (full body DEXA) were performed at 0, 4th and 8th weeks. Myostatin and GASP-1 was measured. Resistance training caused significant decrease in serum levels of myostatin and increase in that of GASP-1. Creatine supplementation in conjunction with resistance training lead to greater decreases in serum myostatin (p<0.05), but had not additional effect on GASP-1 (p>0.05). The effects of resistance training on serum levels of myostatin and GASP-1, may explain the increased muscle mass that is amplified by creatine supplementation.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Administration, Oral
  • Body Composition / drug effects
  • Creatine / administration & dosage*
  • Creatine / pharmacology*
  • Creatine / urine
  • Diet
  • Dietary Supplements
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Male
  • Muscle Strength / drug effects
  • Myostatin / blood*
  • Proteins / metabolism*
  • Resistance Training*
  • Vesicular Transport Proteins / blood*
  • Young Adult


  • Intercellular Signaling Peptides and Proteins
  • Myostatin
  • Proteins
  • Vesicular Transport Proteins
  • WFIKKN2 protein, human
  • Creatine