Microscopic diffusion tensor imaging of the mouse brain

Neuroimage. 2010 Apr 1;50(2):465-71. doi: 10.1016/j.neuroimage.2009.12.057. Epub 2009 Dec 23.


Diffusion tensor imaging (DTI) data at 43 mum isotropic resolution has been acquired on the intact adult mouse brain in 28-h scan time by using a streamlined protocol, including specimen fixation and staining, image acquisition, reconstruction, post-processing, and distribution. An intermediate registration of each component image is required to achieve the desired microscopic resolution. Multiple parameters have been derived, including fractional anisotropy, axial and radial diffusivity, and a color-coded orientation map of the primary eigenvector. Each DTI dataset was mapped to a common reference space to facilitate future standardized analysis. Fiber tracking has also been demonstrated, providing 3D connection information. This protocol to acquire high-resolution DTI data in a robust and repeatable fashion will serve as a foundation to quantitatively study mouse brain integrity and white matter architecture, at what we believe to be the highest spatial resolution yet attained.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anisotropy
  • Brain / anatomy & histology*
  • Brain Mapping / methods*
  • Diffusion Tensor Imaging / methods*
  • Image Interpretation, Computer-Assisted / methods*
  • Mice
  • Staining and Labeling / methods
  • Tissue Fixation / methods