Gene expression profile of protein kinases reveals a distinctive signature in chronic lymphocytic leukemia and in vitro experiments support a role of second generation protein kinase inhibitors

Leuk Res. 2010 Jun;34(6):733-41. doi: 10.1016/j.leukres.2009.11.005. Epub 2009 Dec 24.


To investigate the role of protein kinases (PKs) in chronic lymphocytic leukemia (CLL), we performed gene expression profile on 505 PK genes. Comparison between CLL with acute lymphocytic leukemia (ALL) patients highlighted an homogeneous up-modulation of several PKs in CLL, 16 also overexpressed in two additional CLL cohorts. Q-PCR analysis confirmed these findings. No differences were observed in the main prognostic subclasses, indicating that PK overexpression is specific of the disease itself. Tests in vitro showed that Dasatinib partially reduced CLL cells viability, mostly in IGHV germline patients. These findings suggest that treatment with second generation tyrosine kinase (TK) inhibitors may represent an attractive therapeutic strategy for CLL patients.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adult
  • Aged
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / classification
  • Antineoplastic Agents / therapeutic use
  • Cluster Analysis
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Leukemic
  • Hematologic Neoplasms / genetics
  • Hematologic Neoplasms / metabolism
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / drug therapy
  • Leukemia, Lymphocytic, Chronic, B-Cell / enzymology
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • Leukemia, Lymphocytic, Chronic, B-Cell / metabolism
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Phosphorylation / genetics
  • Phosphorylation / physiology
  • Protein Kinase Inhibitors / chemical synthesis
  • Protein Kinase Inhibitors / classification*
  • Protein Kinase Inhibitors / therapeutic use*
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • Tumor Cells, Cultured
  • ZAP-70 Protein-Tyrosine Kinase / genetics


  • Antineoplastic Agents
  • Protein Kinase Inhibitors
  • Protein Kinases
  • ZAP-70 Protein-Tyrosine Kinase