Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells

Cell Stem Cell. 2010 Jan 8;6(1):71-9. doi: 10.1016/j.stem.2009.12.001. Epub 2009 Dec 31.

Abstract

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascorbic Acid / pharmacology*
  • Cells, Cultured
  • Cellular Reprogramming / drug effects*
  • Cellular Senescence / drug effects
  • Cytological Techniques
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / drug effects
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / drug effects*
  • Mice

Substances

  • Ascorbic Acid

Associated data

  • GEO/GSE19377
  • GEO/GSE19378