Gene expression profiling implicates OXPHOS complexes in lifespan extension of flies over-expressing a small mitochondrial chaperone, Hsp22

Exp Gerontol. 2010 Aug;45(7-8):611-20. doi: 10.1016/j.exger.2009.12.012. Epub 2009 Dec 29.


Aging is a complex process accompanied by a decreased capacity to tolerate and respond to various stresses. Heat shock proteins as part of cell defense mechanisms are up-regulated following stress. In Drosophila, the mitochondrial Hsp22 is preferentially up-regulated in aged flies. Its over-expression results in an extension of lifespan and an increased resistance to stress. Hsp22 has chaperone-like activity in vitro, but the mechanism(s) by which it increases lifespan in flies are unknown. Genome-wide analysis was performed on long-lived Hsp22+ and control flies to unveil transcriptional changes brought by Hsp22. Transcriptomes obtained at 45days, 90% and 50% survival were then compared between them to focus more on genes up- or down-regulated in presence of higher levels of hsp22 mRNA. Hsp22+ flies display an up-regulation of genes mainly related to mitochondrial energy production and protein biosynthesis, two functions normally down-regulated during aging. Interestingly, among the 26 genes up-regulated in Hsp22+ flies, 7 genes encode for mitochondrial proteins, 5 of which being involved in OXPHOS complexes. Other genes that could influence aging such as CG5002, dGCC185 and GstS1 also displayed a regulation linked to Hsp22 expression. The up-regulation of genes of the OXPHOS system in Hsp22+ flies suggest that mitochondrial homeostasis is at the center of Hsp22 beneficial effects on lifespan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • DNA Primers / genetics
  • Drosophila / genetics*
  • Drosophila / growth & development
  • Drosophila / metabolism*
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism*
  • Electron Transport Complex I / genetics
  • Electron Transport Complex I / metabolism
  • Gene Expression Profiling
  • Genes, Insect
  • Genes, Mitochondrial
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / metabolism*
  • Longevity / genetics*
  • Longevity / physiology*
  • Mitochondria / metabolism*
  • NADH Dehydrogenase / genetics
  • NADH Dehydrogenase / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Oxidative Phosphorylation*
  • Up-Regulation


  • DNA Primers
  • Drosophila Proteins
  • Heat-Shock Proteins
  • Hsp22 protein, Drosophila
  • NADH Dehydrogenase
  • Electron Transport Complex I