Bing De Ling, a Chinese herbal formula, inhibits cancer cells growth via p53

Front Biosci (Elite Ed). 2010 Jan 1;2:221-30. doi: 10.2741/e84.

Abstract

Bing De Ling is a Chinese herbal formula that has been used to treat cancer patients for more than a decade. However, the molecular mechanisms behind its anti-tumor efficacy are still elusive. Here, we show that Bing De Ling inhibits cell proliferation in ovarian cancer epithelial cell lines, OV2008 and C13. It induces G1/S arrest in a p53-dependent manner in that this effect is attenuated in OV2008 cells transfected with dominant-negative p53 plasmid. Moreover, we show that Bing De Ling up-regulates p53 transcriptional activities as well as its downstream target genes, such as p21Cip1, MDM2, and MDMX. In addition, Bing De Ling inhibits MDMX-p53 interaction which may result in stabilization and activation of p53. Collectively, our results suggest that the anti-tumor activity of Bing De Ling may be in part due to activation of p53.

MeSH terms

  • Antineoplastic Agents / metabolism
  • Antineoplastic Agents / pharmacology*
  • Cell Cycle / drug effects*
  • Cell Cycle Proteins
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Drugs, Chinese Herbal / metabolism
  • Drugs, Chinese Herbal / pharmacology*
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Immunoblotting
  • Luciferases
  • Nuclear Proteins / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Tetrazolium Salts
  • Thiazoles
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Antineoplastic Agents
  • Cell Cycle Proteins
  • Drugs, Chinese Herbal
  • MDM4 protein, human
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • Tetrazolium Salts
  • Thiazoles
  • Tumor Suppressor Protein p53
  • Luciferases
  • thiazolyl blue