Effects of TGF beta-1 on mouse embryo implantation and expression of H2-D1 and H2-DM

Front Biosci (Elite Ed). 2010 Jan 1;2:351-60. doi: 10.2741/e95.


We investigated the mechanism that TGF-beta1 influences the immuno-environment at maternal-fetal interface and affects embryo implantation, using mouse uterine horn injection model. The expression of MHC I antigen (H2-D1) and the chaperone of MHC II antigen (H2-DM) after anti-TGF-beta1 antibody or hrTGF-beta1 treated pregnant mice were examined by real-time PCR, western blotting and immunohistochemistry. The results showed that the number of implanted embryos of anti-TGF-beta1 antibody-treated mice was decreased compared with the control. The expression of H2-D1 and H2-DM on days 6 and 7 treated uteri was increased both at mRNA and protein levels. In hrTGF-beta1 treated group, the expression of H2-D1 and H2-DM protein was decreased, and the number of implanted embryos was slightly increased. Immunohistochemical studies revealed that H2-D1 and H2-DM were mainly localized to the primary decidual zone. The anti-TGF-beta1 antibody and exogenous hrTGF-beta1 treatment altered the intensity of H2-D1 and H2-DM signal but did not change their localization. These observations suggested that injection of anti-TGF-beta1 antibody affected the number of mouse embryo implantation, and regulated the expression of H2-DM and H2-Q10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / pharmacology
  • Blotting, Western
  • Embryo Implantation / drug effects*
  • Female
  • Gene Expression Regulation, Developmental / drug effects*
  • H-2 Antigens / metabolism*
  • Immunohistochemistry
  • Mice
  • Pregnancy
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transforming Growth Factor beta1 / immunology
  • Transforming Growth Factor beta1 / metabolism*


  • Antibodies
  • H-2 Antigens
  • Transforming Growth Factor beta1