Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol

Biotechniques. 1991 Jan;10(1):35-6, 38-9.

Abstract

We report an efficient procedure to synthesize either single- or double-stranded probes labeled with biotin-11-dUTP, biotin-21-dUTP or digoxigenin-11-dUTP. To produce the single-stranded probe, only a single primer is utilized in a Taq polymerase amplification of 55 cycles. A cytomegalovirus probe is presented. This procedure allows easy production of nonradioactively labeled pure single-stranded probes of any desired length and specificity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Technical Report

MeSH terms

  • Base Sequence
  • DNA Probes*
  • DNA, Single-Stranded / isolation & purification*
  • DNA-Directed DNA Polymerase / metabolism*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Taq Polymerase

Substances

  • DNA Probes
  • DNA, Single-Stranded
  • Taq Polymerase
  • DNA-Directed DNA Polymerase