Efficiency of hyaluronic acid (HA) sperm selection

J Assist Reprod Genet. 2010 Jan;27(1):13-6. doi: 10.1007/s10815-009-9380-0. Epub 2009 Dec 30.


Purpose: Hyaluronic Acid (HA) has a role as "physiologic selector" for spermatozoa prior to intracytoplasmic sperm injection (ICSI). The objective of this study is to analyze the results achievable by the introduction of a routine HA-ICSI programme.

Methods: We retrospectively observed 293 couples treated with HA-ICSI versus 86 couples treated with conventional PVP-ICSI (historical control group). ICSI was performed on a limited number of oocytes per patient (1-3) according to Italian IVF law at the time of the study. Main outcome measures observed were: fertilization, embryo quality, implantation and pregnancy.

Results: This study showed that Injection of HA-bound spermatozoa (HA-ICSI) significantly improves embryo quality and implantation.

Conclusions: If wider multi-center randomized studies will confirm these beneficial effects on ICSI outcome, HA could be considered as a routine choice for "physiologic" sperm selection prior to ICSI.

MeSH terms

  • Embryo Implantation
  • Female
  • Humans
  • Hyaluronic Acid / therapeutic use*
  • Infertility, Female / therapy*
  • Male
  • Ovulation Induction
  • Retrospective Studies
  • Sperm Injections, Intracytoplasmic / methods
  • Sperm Retrieval*
  • Spermatozoa / physiology*
  • Statistics, Nonparametric
  • Treatment Outcome


  • Hyaluronic Acid