Matrix metalloproteinase-9 reversibly affects the time course of NMDA-induced currents in cultured rat hippocampal neurons

Hippocampus. 2010 Oct;20(10):1105-8. doi: 10.1002/hipo.20736.

Abstract

Matrix Metalloproteinase 9 (MMP-9) has been demonstrated to play a crucial role in maintenance of NMDA receptor-dependent LTP and in lateral mobility of these receptors. However, the effect of MMP-9 on NMDA receptor (NMDAR) functional properties is unknown. For this purpose we have investigated the impact of recombinant MMP-9 on the whole-cell NMDAR-mediated current responses in cultured hippocampal neurons. Treatment with MMP-9 induced a reversible acceleration of desensitization and deactivation kinetics but had no effect on current amplitude. Interestingly, phorbol ester, a PKC activator known to enhance NMDAR lateral mobility, induced kinetic changes of currents similar to those produced by MMP-9. In conclusion, our results show that MMP-9 reversibly modulates the NMDAR kinetics and raise a possibility that this modulation could be related to the lateral mobility of these receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Hippocampus / cytology*
  • Hippocampus / drug effects
  • Hippocampus / enzymology*
  • Long-Term Potentiation / drug effects
  • Long-Term Potentiation / physiology
  • Matrix Metalloproteinase 9 / pharmacology
  • Matrix Metalloproteinase 9 / physiology*
  • Neurons / drug effects
  • Neurons / enzymology*
  • Rats
  • Receptors, N-Methyl-D-Aspartate / drug effects
  • Receptors, N-Methyl-D-Aspartate / physiology*

Substances

  • Receptors, N-Methyl-D-Aspartate
  • Matrix Metalloproteinase 9