[Anti-aging research using SMP30/GNL knockout mice]

Yakugaku Zasshi. 2010 Jan;130(1):25-8. doi: 10.1248/yakushi.130.25.
[Article in Japanese]


Senescence Marker Protein-30 (SMP30) was originally identified as a novel protein in the rat liver, the expression of which decreases with aging. Recently, we identified SMP30 as the lactone-hydrolyzing enzyme gluconolactonases (GNL) of animal species. GNL was a key enzyme which involved in vitamin C biosynthesis, and the essential role of SMP30 in this synthetic process was verified by a nutritional study. SMP30 knockout mice developed symptoms of scurvy when fed a vitamin C-deficient diet, verifying the pivotal role of SMP30 in vitamin C biosynthesis. Moreover, SMP30 knockout mice were shorter in life span than the wild type when fed autoclaved mouse chow contained approximately 55 mg/kg of vitamin C, which we now know contains too little vitamin C to maintain normal levels of vitamin C in tissues. These results demonstrate that vitamin C deficiency shortens longevity, that is, vitamin C deficiency accelerates aging.

Publication types

  • English Abstract
  • Review

MeSH terms

  • Aging* / genetics
  • Animals
  • Ascorbic Acid / biosynthesis*
  • Ascorbic Acid Deficiency / etiology
  • Calcium-Binding Proteins* / physiology
  • Carboxylic Ester Hydrolases* / physiology
  • Humans
  • Intracellular Signaling Peptides and Proteins* / physiology
  • Mice
  • Mice, Knockout*


  • Calcium-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Rgn protein, mouse
  • Carboxylic Ester Hydrolases
  • gluconolactonase
  • Ascorbic Acid