Integrating carbon nanotubes and lipid bilayer for biosensing

Biosens Bioelectron. 2010 Mar 15;25(7):1834-7. doi: 10.1016/j.bios.2009.12.011. Epub 2009 Dec 23.


Membrane proteins, which are the target of most drugs, are implicated in many critical cellular functions such as signal transduction, bioelectricity, exocytosis and endocytosis. Therefore, developing techniques to investigate the functions of membrane proteins is obviously important. Here, we have developed a novel system by integrating artificial lipid bilayer (biomimetic membrane) with single-walled carbon nanotube networks (SWNT-net) based field-effect transistor (FET), and demonstrated that such hybrid nanoelectronic biosensors can specifically and electronically detect the presence and dynamic activities of ionophores (specifically, gramicidin and calcimycin) in their native lipid environment. This technique can potentially be used to examine other membrane proteins (e.g. ligand-gated ion channels, receptors, membrane insertion toxins, and antibacterial peptides) for the purposes of biosensing, fundamental studies, or high throughput drug screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomimetic Materials / chemistry*
  • Biosensing Techniques / methods*
  • Conductometry / methods*
  • Lipid Bilayers / chemistry*
  • Membrane Proteins / chemistry*
  • Nanotechnology / methods*
  • Nanotubes, Carbon / chemistry*
  • Systems Integration


  • Lipid Bilayers
  • Membrane Proteins
  • Nanotubes, Carbon