A L-cysteine sensor based on Pt nanoparticles/poly(o-aminophenol) film on glassy carbon electrode

Bioelectrochemistry. 2010 Aug;79(1):84-9. doi: 10.1016/j.bioelechem.2009.12.003. Epub 2009 Dec 11.

Abstract

Platinum nanoparticles (nano-Pt) and poly(o-aminophenol) (POAP) were fabricated onto the glassy carbon electrode(GCE) to form the nano-Pt/POAP/GCE for the electrochemical determination of L-cysteine. The POAP film was obtained through electrochemical polymerization of o-aminophenol on GCE. The nano-Pt was electrochemically deposited onto the surface of the activated POAP/GCE The resultant nano-Pt/POAP/GCE was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and showed excellent electrochemical response to L-cysteine at low oxidative potential in Britton-Robinson (BR) buffer solution (pH=3.0), with good stability and sensitivity, and featured with a low detection limit (0.08 microM, signal/noise=3) and wide linear range (0.4 microM-6.3mM).The mechanism for the electrochemical oxidation of L-cysteine on the nano-Pt/POAP/GCE was also investigated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon / chemistry*
  • Chemistry Techniques, Analytical / instrumentation*
  • Cysteine / analysis*
  • Cysteine / chemistry
  • Electrochemistry
  • Electrodes
  • Glass / chemistry*
  • Hydrogen-Ion Concentration
  • Limit of Detection
  • Linear Models
  • Metal Nanoparticles / chemistry*
  • Oxidation-Reduction
  • Platinum / chemistry*
  • Polymers / chemistry*
  • Sodium Dodecyl Sulfate / chemistry
  • Surface Properties

Substances

  • Polymers
  • poly(2-aminophenol)
  • Sodium Dodecyl Sulfate
  • Platinum
  • Carbon
  • Cysteine