Abstract
We investigated the effects of non-steroidal anti-inflammatory drugs on substrate-induced currents of L-glutamate (L-Glu) transporter EAAT1 expressed in Xenopus laevis oocytes. Niflumic acid (NFA) and diclofenac inhibited L-Glu-induced current through EAAT1 in a non-competitive manner. NFA produced a leftward shift in reversal potential (E(rev)) of L-Glu-induced current and increased current amplitude at the potentials more negative than -100 mV. Diclofenac had no effects on E(rev) and inhibited the current amplitude to the same extent at all negative potentials. These results indicate that NFA and diclofenac inhibit the L-Glu-induced EAAT1 current via different mechanisms.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / adverse effects
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Anti-Inflammatory Agents, Non-Steroidal / blood
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Diclofenac / adverse effects
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Diclofenac / pharmacology*
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Excitatory Amino Acid Transporter 1 / antagonists & inhibitors*
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Excitatory Amino Acid Transporter 1 / metabolism
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Female
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Humans
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Niflumic Acid / adverse effects
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Niflumic Acid / pharmacology*
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Oocytes / drug effects
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Oocytes / metabolism
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Xenopus laevis
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Excitatory Amino Acid Transporter 1
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SLC1A3 protein, human
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Diclofenac
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Niflumic Acid