Microassay for DNA methyltransferase

J Biochem Biophys Methods. 1991 Jan;22(1):19-22. doi: 10.1016/0165-022x(91)90077-a.

Abstract

A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Modification Methylases / analysis*
  • Kinetics
  • Microchemistry / methods
  • Substrate Specificity

Substances

  • DNA Modification Methylases