Conversion of borate-based glass scaffold to hydroxyapatite in a dilute phosphate solution

Biomed Mater. 2010 Feb;5(1):15005. doi: 10.1088/1748-6041/5/1/015005. Epub 2010 Jan 7.


Porous scaffolds of a borate-based glass (composition in mol%: 6Na2O, 8K2O, 8MgO, 22CaO, 36B2O3, 18SiO2, 2P2O5), with interconnected porosity of approximately 70% and pores of size 200-500 microm, were prepared by a polymer foam replication technique. The degradation of the scaffolds and conversion to a hydroxyapatite-type material in a 0.02 M K2HPO4 solution (starting pH = 7.0) at 37 degrees C were studied by measuring the weight loss of the scaffolds, as well as the pH and the boron concentration of the solution. X-ray diffraction, scanning electronic microscopy and energy dispersive x-ray analysis showed that a hydroxyapatite-type material was formed on the glass surface within 7 days of immersion in the phosphate solution. Cellular response to the scaffolds was assessed using murine MLO-A5 cells, an osteogenic cell line. Scanning electron microscopy showed that the scaffolds supported cell attachment and proliferation during the 6 day incubation. The results indicate that this borate-based glass could provide a promising degradable scaffold material for bone tissue engineering applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Substitutes / chemical synthesis*
  • Borates / chemistry*
  • Cell Line
  • Cell Proliferation
  • Cell Survival
  • Crystallization / methods
  • Durapatite / chemistry*
  • Glass / chemistry
  • Materials Testing
  • Mice
  • Osteoblasts / cytology
  • Osteoblasts / physiology*
  • Phosphates / chemistry*
  • Solutions
  • Surface Properties
  • Tissue Engineering / methods*
  • Tissue Scaffolds*


  • Bone Substitutes
  • Borates
  • Phosphates
  • Solutions
  • Durapatite