Abstract
Previous work has shown that optimal ex vivo expansion and differentiation of CD34(+) progenitor cells into neutrophils is by addition of Flt3-L, SCF and G-CSF. Here we report that a variety of genes involved in the WNT pathway are transcriptionally active in both undifferentiated and differentiated umbilical cord blood CD34(+) cells, however statistically significant changes in gene expression are not always consistent across UCB samples.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD34 / analysis
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Cell Differentiation / drug effects
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Cell Differentiation / genetics*
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Cells, Cultured / cytology
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Cells, Cultured / metabolism
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DNA, Complementary / genetics
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Fetal Blood / cytology*
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Frizzled Receptors / genetics*
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Gene Expression Profiling*
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Hematopoietic Cell Growth Factors / pharmacology
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / drug effects
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Hematopoietic Stem Cells / metabolism*
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Humans
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Infant, Newborn
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Neutrophils / cytology*
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Polymerase Chain Reaction / methods
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RNA, Messenger / genetics
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Transcription, Genetic
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Wnt Proteins / genetics*
Substances
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Antigens, CD34
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DNA, Complementary
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Frizzled Receptors
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Hematopoietic Cell Growth Factors
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RNA, Messenger
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Wnt Proteins