Comparison between three adjuvants for a vaccine against canine leishmaniasis: In vitro evaluation of macrophage killing ability

T Trotta; A Fasanella; D Scaltrito; L Gradoni; V Mitolo; O Brandonisio; A Acquafredda; M A Panaro

doi:10.1016/j.cimid.2009.12.003

Comparison between three adjuvants for a vaccine against canine leishmaniasis: In vitro evaluation of macrophage killing ability

Comp Immunol Microbiol Infect Dis. 2010 Mar;33(2):175-82. doi: 10.1016/j.cimid.2009.12.003. Epub 2010 Jan 12.

Authors

T Trotta¹, A Fasanella, D Scaltrito, L Gradoni, V Mitolo, O Brandonisio, A Acquafredda, M A Panaro

Affiliation

¹ Dipartimento di Scienze Biomediche, University of Foggia, Ospedali Riuniti, Italy. t.trotta@unifg.it

PMID: 20061026
DOI: 10.1016/j.cimid.2009.12.003

Abstract

The aim of this study was to evaluate, in terms of dog macrophage killing ability in vitro, a vaccine based on Leishmania infantum promastigote soluble antigen (LSA) formulated with three different adjuvants (BCG, AdjuPrime, MPL/TDM/CWS). A significant increase of the macrophage killing ability was observed in dogs vaccinated with LSA+MPL/TDM/CWS after 1 month from vaccination. A similar increase of macrophage parasitocidal ability was present only after 5 months in dogs vaccinated with LSA+BCG or LSA+AdjuPrime. In all dogs the augmented killing percentage was still present after 12 months from vaccination. Therefore, in particular LSA+MPL/TDM/CWS vaccine seems promising for further studies in dogs.

Publication types

Clinical Trial, Phase I
Comparative Study
Randomized Controlled Trial

MeSH terms

Adjuvants, Immunologic / therapeutic use*
Animals
Antigens, Protozoan / immunology
BCG Vaccine / immunology
BCG Vaccine / therapeutic use
Cell Wall Skeleton / immunology
Cell Wall Skeleton / therapeutic use
Cord Factors / immunology
Cord Factors / therapeutic use
Dog Diseases / immunology*
Dog Diseases / parasitology
Dog Diseases / prevention & control
Dogs
Female
Humans
Leishmania infantum / immunology*
Leishmaniasis / immunology
Leishmaniasis / parasitology
Leishmaniasis / prevention & control
Leishmaniasis / veterinary*
Leishmaniasis Vaccines / immunology
Leishmaniasis Vaccines / therapeutic use*
Leukocytes, Mononuclear / immunology
Lipid A / analogs & derivatives
Lipid A / immunology
Lipid A / therapeutic use
Macrophages / immunology*
Male
Time Factors

Substances

Adjuvants, Immunologic
Antigens, Protozoan
BCG Vaccine
Cell Wall Skeleton
Cord Factors
Leishmaniasis Vaccines
Lipid A
6,6'-dicorynomycolyl trehalose
monophosphoryl lipid A