Effect of extract of Withania Somnifera on dehydration-induced oxidative stress-related uremia in male rats

Saudi J Kidney Dis Transpl. 2010 Jan;21(1):75-80.


Dehydration or water deprivation in the body decreases urinary excretion and allows urea and other protein waste products to accumulate in the blood. The aim of the present study is to evaluate the association of uremia and oxidative stress by applying the herbal plant Withania somnifera (W. somnifera) (Aswagandha). The study was performed on male Wister strain rats in which, dehydration was achieved by water withdrawal. A total of 18 rats were studied and were randomly divided into three Groups: Group-1, control, Group-2, only dehydration and Group-3, dehydration + administration of aqueous root extract of W. somnifera, orally (50 mg/100 gm body weight/day) for 25 days. After 25 days of treatment, it was observed that the body weight of Group-3 animals had increased significantly, while that in Group-2 had decreased significantly. The liver enzymes in both blood and kidneys did not show any significant change in the three groups implying absence of any toxicity of the root extract. In Group-2 animals, the serum urea and creatinine levels increased sig-nificantly when compared with animals in Groups-1 and 3. The low levels of serum urea and crea-tinine in Group-3 animals indicates the protective effect of the plant extract against renal injury caused by dehydration. Dehydration-induced oxidative stress was established in our study by noting the low activities of super-oxide dismutase and catalase, both important antioxidant enzymes, in Group-2 animals; both enzymes were stabilized in animals of Groups-3 and 1. In conclusion, it is hypothesized that there is an antioxidative role of W. somnifera resulting in reducing the extent of renal injury as a result of oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Alanine Transaminase / blood
  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology*
  • Aspartate Aminotransferases / blood
  • Biomarkers / blood
  • Body Weight
  • Catalase / blood
  • Creatinine / blood
  • Dehydration / complications
  • Dehydration / drug therapy*
  • Dehydration / metabolism
  • Disease Models, Animal
  • Kidney / drug effects*
  • Kidney / enzymology
  • Kidney / metabolism
  • Male
  • Oxidative Stress / drug effects*
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Plant Roots
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / blood
  • Time Factors
  • Urea / blood
  • Uremia / etiology
  • Uremia / metabolism
  • Uremia / prevention & control*
  • Water Deprivation
  • Withania*


  • Antioxidants
  • Biomarkers
  • Plant Extracts
  • Urea
  • Creatinine
  • Catalase
  • Superoxide Dismutase
  • Aspartate Aminotransferases
  • Alanine Transaminase