Abstract
We report that the activity of a rationally engineered protein tyrosine phosphatase (PTP) mutant can be fully rescued by the addition of the biarsenical fluorescein derivative FlAsH, a compound that does not affect the activity of wild-type PTPs.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Catalytic Domain
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Enzyme Activation*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Fluoresceins / metabolism*
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Gene Expression
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Mutation
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Protein Binding
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Protein Tyrosine Phosphatase, Non-Receptor Type 12 / chemistry
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Protein Tyrosine Phosphatase, Non-Receptor Type 12 / genetics*
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Protein Tyrosine Phosphatase, Non-Receptor Type 12 / metabolism*
Substances
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Fluoresceins
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Protein Tyrosine Phosphatase, Non-Receptor Type 12