Chemical rescue of protein tyrosine phosphatase activity

Chem Commun (Camb). 2010 Jan 28;46(4):637-9. doi: 10.1039/b919815f. Epub 2009 Nov 18.

Abstract

We report that the activity of a rationally engineered protein tyrosine phosphatase (PTP) mutant can be fully rescued by the addition of the biarsenical fluorescein derivative FlAsH, a compound that does not affect the activity of wild-type PTPs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalytic Domain
  • Enzyme Activation*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Fluoresceins / metabolism*
  • Gene Expression
  • Mutation
  • Protein Binding
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12 / chemistry
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12 / genetics*
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12 / metabolism*

Substances

  • Fluoresceins
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12