Lights on iron-sulfur clusters

Chem Biol. 2009 Dec 24;16(12):1213-4. doi: 10.1016/j.chembiol.2009.12.005.

Abstract

The biogenesis of iron-sulfur proteins within a living cell is traditionally investigated by enzyme activity measurements and radiolabeling techniques. In this issue of Chemistry and Biology, Hoff et al. introduce a non-invasive fluorescence-based method to trace the formation of iron-sulfur clusters at high sensitivity.

Publication types

  • Comment

MeSH terms

  • Cell Line
  • Dimerization
  • Glutaredoxins / chemistry
  • Glutaredoxins / genetics
  • Glutaredoxins / metabolism*
  • Humans
  • Iron / chemistry*
  • Luminescent Proteins / chemistry
  • Luminescent Proteins / metabolism
  • Microscopy, Fluorescence
  • Mutation
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sulfur / chemistry*

Substances

  • GLRX2 protein, human
  • Glutaredoxins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Sulfur
  • Iron