The Role of Cytotoxic and Regulatory T Cells in relapsed/refractory Hodgkin Lymphoma

Appl Immunohistochem Mol Morphol. 2010 May;18(3):206-11. doi: 10.1097/PAI.0b013e3181c7138b.


Recent data suggests the presence of cytotoxic (TIA-1 and granzyme B+) and regulatory T-cells (FOXP3+) in classical Hodgkin lymphoma (cHL) tissues has been shown to correlate with poor overall survival in mainly diagnostic biopsies. By tissue microarray analyses, we extend this observation to a cohort of relapsed/refractory cHL tissue biopsies and analyze immunohistochemical expression of FOXP3, TIA-1, and granzyme B in the inflammatory background and the tumor microenvironment. High expression of TIA-1 (>50%) correlated with poor overall survival (P<0.0001), low expression of FOXP3 (<25%) correlated with poor overall survival (P<0.01), and combined high TIA-1 (>50%) and low FOXP3 (<25%) correlated with poor overall survival (P<0.0001). Expression of cytotoxic and regulatory T-cells shows prognostic significance in the relapsed/refractory clinical setting of cHL.

MeSH terms

  • Adolescent
  • Adult
  • Biopsy
  • Drug Resistance, Neoplasm
  • Female
  • Forkhead Transcription Factors / metabolism*
  • Granzymes / metabolism*
  • Hodgkin Disease / diagnosis
  • Hodgkin Disease / immunology*
  • Hodgkin Disease / mortality
  • Hodgkin Disease / pathology*
  • Hodgkin Disease / physiopathology
  • Humans
  • Immunochemistry
  • Male
  • Microarray Analysis
  • Middle Aged
  • Poly(A)-Binding Proteins / metabolism*
  • Predictive Value of Tests
  • Prognosis
  • Recurrence
  • Survival Analysis
  • T-Cell Intracellular Antigen-1
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Cytotoxic / metabolism*
  • T-Lymphocytes, Cytotoxic / pathology
  • T-Lymphocytes, Regulatory / metabolism*
  • T-Lymphocytes, Regulatory / pathology


  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Poly(A)-Binding Proteins
  • T-Cell Intracellular Antigen-1
  • TIA1 protein, human
  • Granzymes