Abstract
Ectopic expression of coupling factor B in animal cells resulted in altered mitochondrial morphology. Cells expressing factor B fused to green fluorescent protein (GFP) contained fragmented, balloon-shaped or thinned, filamentous mitochondria, terminating at one end with balloon-like structures. Ultrastructural analysis using transmission electron microscopy revealed changes in the organization of mitochondrial cristae in cells expressing factor B-GFP fusion protein.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenosine Triphosphate / biosynthesis
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Animals
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Cell Line
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Energy Metabolism
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HeLa Cells
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Humans
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LLC-PK1 Cells
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Membrane Potential, Mitochondrial
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Mice
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Microscopy, Confocal
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Microscopy, Electron, Transmission
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Mitochondria / metabolism*
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Mitochondria / ultrastructure*
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Mitochondrial Membranes / metabolism
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Mitochondrial Membranes / ultrastructure
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Mitochondrial Proton-Translocating ATPases / genetics
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Mitochondrial Proton-Translocating ATPases / metabolism*
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Oxidative Phosphorylation Coupling Factors / genetics
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Oxidative Phosphorylation Coupling Factors / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Submitochondrial Particles / metabolism
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Submitochondrial Particles / ultrastructure
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Swine
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Transfection
Substances
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Oxidative Phosphorylation Coupling Factors
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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Adenosine Triphosphate
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coupling factor B
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Mitochondrial Proton-Translocating ATPases