Coupling factor B affects the morphology of mitochondria

J Bioenerg Biomembr. 2010 Feb;42(1):29-35. doi: 10.1007/s10863-009-9263-1. Epub 2010 Jan 13.

Abstract

Ectopic expression of coupling factor B in animal cells resulted in altered mitochondrial morphology. Cells expressing factor B fused to green fluorescent protein (GFP) contained fragmented, balloon-shaped or thinned, filamentous mitochondria, terminating at one end with balloon-like structures. Ultrastructural analysis using transmission electron microscopy revealed changes in the organization of mitochondrial cristae in cells expressing factor B-GFP fusion protein.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenosine Triphosphate / biosynthesis
  • Animals
  • Cell Line
  • Energy Metabolism
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HeLa Cells
  • Humans
  • LLC-PK1 Cells
  • Membrane Potential, Mitochondrial
  • Mice
  • Microscopy, Confocal
  • Microscopy, Electron, Transmission
  • Mitochondria / metabolism*
  • Mitochondria / ultrastructure*
  • Mitochondrial Membranes / metabolism
  • Mitochondrial Membranes / ultrastructure
  • Mitochondrial Proton-Translocating ATPases / genetics
  • Mitochondrial Proton-Translocating ATPases / metabolism*
  • Oxidative Phosphorylation Coupling Factors / genetics
  • Oxidative Phosphorylation Coupling Factors / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Submitochondrial Particles / metabolism
  • Submitochondrial Particles / ultrastructure
  • Swine
  • Transfection

Substances

  • Oxidative Phosphorylation Coupling Factors
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Adenosine Triphosphate
  • coupling factor B
  • Mitochondrial Proton-Translocating ATPases