Abstract
2(E),4(E)-Decadienal (DDE), a lipid peroxidation product, was found to covalently modify Lys residues of different proteins by different reactions using mass spectrometry (MALDI-TOF-MS and LC-ESI-MS). DDE mainly formed Lys Schiff base adducts with cytochrome c and ribonuclease A at 10 min, but these reversibly formed adducts almost disappeared after 24 h. In contrast, beta-lactoglobulin (beta-LG) was highly modified by DDE after 24 h. In addition to the Lys Schiff base adducts, DDE formed novel Lys pyridinium adducts as well as Cys Michael adducts with beta-LG.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Aldehydes / adverse effects*
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Amino Acid Sequence
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Cytochromes c / analysis
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Cytochromes c / metabolism
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Lactoglobulins / analysis
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Lactoglobulins / metabolism
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Mass Spectrometry*
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Molecular Sequence Data
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Proteins / analysis
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Proteins / metabolism*
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Ribonuclease, Pancreatic / analysis
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Ribonuclease, Pancreatic / metabolism
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Spectrometry, Mass, Electrospray Ionization
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Aldehydes
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Lactoglobulins
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Proteins
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2,4-decadienal
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Cytochromes c
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Ribonuclease, Pancreatic