P 53 Codon 72 Polymorphism in Stomach and Colorectal Adenocarcinomas in Iranian Patients

Indian J Cancer. Jan-Mar 2010;47(1):31-4. doi: 10.4103/0019-509X.58856.

Abstract

Background: The association of a functional single nucleotide polymorphism at codon 72 of the p53 gene (Arg72Pro) with malignancy is a subject of controversy. We analyzed this polymorphism in 224 patients with gastrointestinal cancers (92 with stomach cancer and 132 with colorectal cancer) and in 163 healthy controls.

Material and methods: DNA was extracted from peripheral blood mononuclear cells and amplified with an allele-specific polymerase chain reaction.

Results: There was no significant association between p53 alleles and gastrointestinal cancers. The frequency of the Arg allele was 59.7, 58.8, and 59.2% in the stomach cancer patients, colorectal cancer patients, and controls, respectively. Frequencies of the Pro allele were 40.3% in patients with stomach cancer, 41.2% in patients with colorectal cancer, and 40.8% in controls. Likewise, genotype frequencies did not differ significantly between the two patient groups and controls. There were no differences in genotype or allele frequencies by gender, age, or histological grade.

Conclusions: The data do not support the association of the p53 codon 72 polymorphism with stomach or colorectal cancers in Iranian patients.

MeSH terms

  • Adenocarcinoma / genetics*
  • Colorectal Neoplasms / genetics*
  • Female
  • Genetic Predisposition to Disease*
  • Genotype
  • Humans
  • Iran
  • Male
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Stomach Neoplasms / genetics*
  • Tumor Suppressor Protein p53 / genetics*

Substances

  • Tumor Suppressor Protein p53