Function of endoplasmic reticulum calcium ATPase in innate immunity-mediated programmed cell death

EMBO J. 2010 Mar 3;29(5):1007-18. doi: 10.1038/emboj.2009.402. Epub 2010 Jan 14.

Abstract

Programmed cell death (PCD) initiated at the pathogen-infected sites during the plant innate immune response is thought to prevent the development of disease. Here, we describe the identification and characterization of an ER-localized type IIB Ca(2+)-ATPase (NbCA1) that function as a regulator of PCD. Silencing of NbCA1 accelerates viral immune receptor N- and fungal-immune receptor Cf9-mediated PCD, as well as non-host pathogen Pseudomonas syringae pv. tomato DC3000 and the general elicitor cryptogein-induced cell death. The accelerated PCD rescues loss-of-resistance phenotype of Rar1, HSP90-silenced plants, but not SGT1-silenced plants. Using a genetically encoded calcium sensor, we show that downregulation of NbCA1 results in the modulation of intracellular calcium signalling in response to cryptogein elicitor. We further show that NbCAM1 and NbrbohB function as downstream calcium decoders in N-immune receptor-mediated PCD. Our results indicate that ER-Ca(2+)-ATPase is a component of the calcium efflux pathway that controls PCD during an innate immune response.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Apoptosis / genetics
  • Apoptosis / physiology*
  • Calcium-Transporting ATPases / genetics
  • Calcium-Transporting ATPases / metabolism*
  • Endoplasmic Reticulum / enzymology*
  • Gene Expression Regulation, Plant / genetics
  • Gene Expression Regulation, Plant / physiology
  • Immunity, Innate / genetics
  • Immunity, Innate / physiology*
  • Microscopy, Fluorescence
  • Pseudomonas syringae / growth & development
  • Pseudomonas syringae / immunology
  • Tobacco / enzymology*
  • Tobacco / immunology*
  • Tobacco / microbiology
  • Tobacco / virology
  • Tobacco Mosaic Virus / growth & development
  • Tobacco Mosaic Virus / immunology

Substances

  • Calcium-Transporting ATPases