In this study, we examined the relationship between serum creatine kinase activity following exercise-induced muscle damage and muscle fibre composition. Seventeen untrained males volunteered and underwent a .[Vdot]O2max test, Wingate test, and an exercise-induced muscle damage protocol. Muscle soreness and blood samples were recorded before, immediately after, and 24, 48, 72, and 96 h after exercise. Biopsy samples from the vastus lateralis were collected one week after exercise-induced muscle damage and were assessed for muscle fibre composition. There was no significant relationship (P > 0.05) between muscle fibre composition and creatine kinase activity. A significant positive correlation (P < 0.05) was observed between soreness 48 h after exercise and type II and IIb fibres, and a significant negative correlation (P < 0.05) was observed between soreness 48 h after exercise and type I muscle fibres. Significant positive correlations were observed between soreness 48 h after exercise and the fatigue index, relative average power, and relative anaerobic capacity. Our results suggest that creatine kinase activity following exercise-induced muscle damage may not be related to muscle fibre proportions, and higher post-exercise muscular pain may be related to a predominance of type II muscle fibres and higher anaerobic capabilities.