Sickness-related odor communication signals as determinants of social behavior in rat: a role for inflammatory processes

Horm Behav. 2010 Mar;57(3):330-41. doi: 10.1016/j.yhbeh.2010.01.002. Epub 2010 Jan 14.

Abstract

Infected animals are avoided by conspecifics, suggesting that the inflammatory cascade may play a significant role in odor communication. Injection of male rats with the bacterial mimetic, lipopolysaccharide (LPS, 100 microg/kg, i.p.), decreased investigation through a wire-mesh partition between healthy male partners. This avoidance response was observed in adult males in response to soiled bedding collected from sick rats, regardless of whether LPS was injected peripherally (100 microg/kg, i.p.) or centrally (0.25 or 2.5 microg, icv). The release of sickness-related odor cues was dose-dependently blocked by icv infusion of the anti-inflammatory cytokine, interleukin-10 (IL-10; 20 or 200 ng), and reproduced by icv infusion of pro-inflammatory cytokine, IL-1beta (5 or 50 ng). Subcutaneous pretreatment with either estradiol benzoate (20 microg/kg) or testosterone propionate (50 or 500 microg/kg) to adult males that were administered LPS inhibited release of aversive odor cues, but these hormones alone did not influence odor properties. Importantly, the avoidance response to sickness-related odor was not associated with changes in plasma corticosterone, testosterone, or IL-6 levels of odor donors. However, plasma IL-1beta concentrations of sick animals was in fact predictive of aversive responses in conspecifics, suggesting that the inflammatory cascade, but not plasma steroid hormones, is likely to mediate aversive properties in odor that functions to signal illness state to conspecifics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacterial Infections* / metabolism
  • Corticosterone / blood
  • Cues
  • Estradiol / analogs & derivatives
  • Estradiol / pharmacology
  • Hormones / pharmacology
  • Inflammation* / metabolism
  • Interleukin-10 / metabolism
  • Interleukin-1beta / blood
  • Interleukin-1beta / metabolism
  • Interleukin-6 / blood
  • Lipopolysaccharides / toxicity
  • Male
  • Odorants*
  • Olfactory Perception*
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Social Behavior*
  • Testosterone / blood
  • Testosterone Propionate / pharmacology

Substances

  • Hormones
  • Interleukin-1beta
  • Interleukin-6
  • Lipopolysaccharides
  • Interleukin-10
  • estradiol 3-benzoate
  • Testosterone
  • Estradiol
  • Corticosterone
  • Testosterone Propionate