Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1

Virology. 2010 Mar 15;398(2):290-8. doi: 10.1016/j.virol.2009.12.010. Epub 2010 Jan 15.


The dengue virus (DENV) nonstructural protein 1 (NS1) is an immunogenic protein that holds potential for the development of vaccines and diagnostic reagents; however, the epitopes of NS1 have not been comprehensively mapped. We mapped B-cell linear epitopes on NS1 using 149 monoclonal antibodies with DENV serotype specificity and cross-reactivity as well as antisera from 27 mice immunized with the four DENV serotypes. Epitope recognition analysis was performed using a set of 15-mer sequential overlapping peptides that spanned the entire NS1 protein from DENV-1. This strategy identified three regions of NS1 that are DENV-1 serotype-specific epitopes, namely amino acid residues 1-15, 71-85, and 338-352. We also identified five group-specific B-cell epitopes that were highly conserved among isolates of the four DENV serotypes. These novel immunodominant serotype- and group-specific B-cell epitopes of DENV NS1 may aid the development of new dengue vaccines and diagnostic assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Blotting, Western
  • Cell Line
  • Chromosome Mapping
  • Conserved Sequence / genetics
  • Cross Reactions / genetics
  • Cross Reactions / immunology
  • Dengue Virus / classification
  • Dengue Virus / genetics*
  • Dengue Virus / immunology
  • Epitopes, B-Lymphocyte / genetics*
  • Epitopes, B-Lymphocyte / immunology
  • Immunodominant Epitopes / genetics*
  • Immunodominant Epitopes / immunology
  • Mice
  • Sequence Alignment
  • Serotyping
  • Viral Proteins / genetics
  • Viral Proteins / immunology


  • Antibodies, Monoclonal
  • Epitopes, B-Lymphocyte
  • Immunodominant Epitopes
  • Viral Proteins