Sulfur amino acids in methionine-restricted rats: hyperhomocysteinemia

Nutrition. Nov-Dec 2010;26(11-12):1201-4. doi: 10.1016/j.nut.2009.09.017. Epub 2010 Jan 18.

Abstract

Objective: Dietary methionine restriction in Fischer-344 rats favorably influences visceral fat mass, insulin sensitivity, metabolic parameters, and longevity. However, little is known about the effects of methionine restriction on serum methionine and its downstream sulfur amino acids. We investigated the serum sulfur amino acid profile of male Fischer-344 rats fed a methionine-restricted diet for 3 mo.

Methods and results: Using tandem mass spectrometry, we observed marked reduction in serum concentrations of methionine, cystathionine, cysteine, and taurine in methionine-restricted rats compared with control (P<0.001) and a 2.5-fold elevation of homocysteine (P<0.001).

Conclusion: This suggests that homocysteine trans-sulfuration may be inhibited by methionine restriction, and that some of the effects of methionine restriction may be mediated by changes in sulfur amino acids downstream of methionine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiposity
  • Amino Acids, Sulfur / blood*
  • Amino Acids, Sulfur / chemistry
  • Animals
  • Body Weight
  • Cystathionine / blood
  • Cystathionine / chemistry
  • Cysteine / blood
  • Cysteine / chemistry
  • Diet / adverse effects
  • Homocysteine / blood
  • Homocysteine / chemistry
  • Homocysteine / metabolism
  • Hyperhomocysteinemia / blood*
  • Hyperhomocysteinemia / etiology*
  • Hyperhomocysteinemia / metabolism
  • Intra-Abdominal Fat
  • Male
  • Methionine / blood
  • Methionine / chemistry
  • Methionine / deficiency*
  • Methionine / metabolism*
  • Random Allocation
  • Rats
  • Rats, Inbred F344
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry
  • Taurine / blood
  • Taurine / chemistry

Substances

  • Amino Acids, Sulfur
  • Homocysteine
  • Taurine
  • Cystathionine
  • Methionine
  • Cysteine