JAK-STAT is restrained by Notch to control cell proliferation of the Drosophila intestinal stem cells

J Cell Biochem. 2010 Apr 1;109(5):992-9. doi: 10.1002/jcb.22482.


The Drosophila midgut epithelium undergoes continuous regeneration that is sustained by multipotent intestinal stem cells (ISCs) underneath. Notch signaling has dual functions to control ISC behavior: it slows down the ISC proliferation and drives the activated ISCs into different differentiation pathways at a dose-dependent manner. Here we identified a molecular mechanism to unite these two contradictory functions. We found JAK-STAT signaling controls ISC proliferation and this ability is negatively regulated by Notch at least through a transcriptional control of the JAK-STAT signaling ligand, unpaired (upd). This study provides insight into how stem cells, under steady conditions, balance the processes of proliferation and differentiation to maintain the stable cellular composition of a healthy tissue.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cell Proliferation
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / enzymology*
  • Intestines / cytology*
  • Janus Kinases / metabolism*
  • Receptors, Notch / metabolism*
  • STAT Transcription Factors / metabolism*
  • Signal Transduction*
  • Stem Cells / cytology*
  • Stem Cells / enzymology
  • Transcription, Genetic


  • Drosophila Proteins
  • Receptors, Notch
  • STAT Transcription Factors
  • Janus Kinases