A manually curated network of the PML nuclear body interactome reveals an important role for PML-NBs in SUMOylation dynamics

Int J Biol Sci. 2010 Jan 12;6(1):51-67. doi: 10.7150/ijbs.6.51.


Promyelocytic Leukaemia Protein nuclear bodies (PML-NBs) are dynamic nuclear protein aggregates. To gain insight in PML-NB function, reductionist and high throughput techniques have been employed to identify PML-NB proteins. Here we present a manually curated network of the PML-NB interactome based on extensive literature review including database information. By compiling 'the PML-ome', we highlighted the presence of interactors in the Small Ubiquitin Like Modifier (SUMO) conjugation pathway. Additionally, we show an enrichment of SUMOylatable proteins in the PML-NBs through an in-house prediction algorithm. Therefore, based on the PML network, we hypothesize that PML-NBs may function as a nuclear SUMOylation hotspot.

Keywords: Cytoscape; PML-NB; SUMOylation; network; protein-protein interaction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Algorithms
  • Animals
  • Cell Nucleus / metabolism
  • Humans
  • Models, Biological
  • Nuclear Proteins / metabolism*
  • Promyelocytic Leukemia Protein
  • Protein Multimerization
  • Signal Transduction
  • Small Ubiquitin-Related Modifier Proteins / metabolism*
  • Transcription Factors / metabolism*
  • Tumor Suppressor Proteins / metabolism*
  • Ubiquitination


  • Nuclear Proteins
  • Promyelocytic Leukemia Protein
  • Small Ubiquitin-Related Modifier Proteins
  • Transcription Factors
  • Tumor Suppressor Proteins
  • PML protein, human