A fraction of unripe kiwi fruit extract regulates adipocyte differentiation and function in 3T3-L1 cells

Biofactors. 2010 Jan-Feb;36(1):52-9. doi: 10.1002/biof.70.


Adipocyte dysfunction is strongly associated with the development of insulin resistance and diabetes, and regulation of adipogenesis is important in prevention of diabetes. We prepared a 100% methanol fraction of methanolic extract from unripe kiwi fruit (Actinidia deliciosa), designated KMF (kiwi fruit methanol fraction). When applied to 3T3-L1 preadipocyte cells, KMF promoted adipocyte differentiation, increased glycerol-3-phosphate dehydrogenase (GPDH) activity, and increased triglyceride (TG) content. KMF markedly increased mRNA expression of peroxisome proliferator-activated receptor gamma (PPARgamma)-the master adipogenic transcription factor-and its target genes. Moreover, KMF increased mRNA expression and protein secretion of adiponectin, whereas mRNA expression and secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) were decreased. Compared with troglitazone, KMF decreased the production of reactive oxygen species (ROS) and nuclear factor-kappaB (NFkappaB) activation. Glucose uptake was stimulated by KMF in differentiated 3T3-L1 adipocytes. Taken together, these results indicate that KMF may exert beneficial effects against diabetes via its ability to regulate adipocyte differentiation and function.

MeSH terms

  • 3T3-L1 Cells
  • Actinidia / chemistry*
  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipocytes / physiology*
  • Adipogenesis / drug effects
  • Adiponectin / biosynthesis
  • Animals
  • Cell Differentiation / drug effects
  • Chemokine CCL2 / metabolism
  • Chromans / pharmacology
  • Glucose / metabolism
  • Glycerolphosphate Dehydrogenase / metabolism
  • Interleukin-6 / metabolism
  • Mice
  • NF-kappa B / physiology
  • Plant Extracts / pharmacology*
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism
  • Thiazolidinediones / pharmacology
  • Triglycerides / metabolism
  • Troglitazone


  • Adiponectin
  • Ccl2 protein, mouse
  • Chemokine CCL2
  • Chromans
  • Interleukin-6
  • NF-kappa B
  • Plant Extracts
  • RNA, Messenger
  • Reactive Oxygen Species
  • Thiazolidinediones
  • Triglycerides
  • Glycerolphosphate Dehydrogenase
  • Troglitazone
  • Glucose