Construction of aromatic dependent Shigella flexneri 2a live vaccine candidate strains: deletion mutations in the aroA and the aroD genes

Vaccine. 1991 Jan;9(1):6-9. doi: 10.1016/0264-410x(91)90308-s.

Abstract

Live Shigella flexneri 2a vaccine candidate strains with deletion mutations in the aro genes were constructed. Tn10-generated auxotrophic mutations were transduced from Escherichia coli to S. flexneri 2a with bacteriophage P1CmCts. The tetracycline-sensitive derivatives of Tn10 mutants obtained were selected on Bochner's medium and checked by DNA-DNA hybridization using aroA and aroD gene specific probes. The vaccine candidate strains were tested to assess the efficacy of protection in guinea-pig conjunctival epithelia (Sereny test). The strains did not cause keratoconjunctivitis and exhibited significant protection in the challenge experiments. A candidate vaccine strain (delta aroD) showed 100% protection against 10(7) c.f.u. of wild type strain in the immunized guinea-pigs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Vaccines / isolation & purification*
  • Bacterial Vaccines / therapeutic use
  • Chromosome Deletion
  • Dysentery, Bacillary / prevention & control
  • Female
  • Guinea Pigs
  • Immunization
  • Mutation
  • Shigella flexneri / genetics
  • Shigella flexneri / immunology*

Substances

  • Bacterial Vaccines